Description
Optical projection tomography (OPT) is in many ways the optical equivalent of x-ray computed tomography (CT) or the medical CT scan. OPT differs in the way that it often uses ultraviolet, visible, and near-infrared photons as opposed to X-ray photons. However, essential mathematics and reconstruction algorithms used for CT and OPT are similar; for example, radon transform or iterative reconstruction, based on projection data are used in both medical CT scan and OPT for 3D reconstruction.

Service provider
EMBL-Barcelona 

Location
Barcelona, Spain

Contacts
Scientific contact: Jim Swoger  
Technical contact: Jim Swoger

Description
Light sheet microscopy, often referred to as single plane illumination microscopy (SPIM), is a rapidly emerging technology that combines optical sectioning with multiple-view imaging to observe tissues and living organisms with impressive resolution. Unlike the conventional techniques of widefield and confocal fluorescence microscopy, the light sheet technique illuminates only the region surrounding the focal plane of the detection objective in a twin objective configuration.

Service provider I
EMBL-Barcelona

Location
Barcelona, Spain

Contacts
Scientific contact: Jim Swoger 
Technical contact: Jim Swoger

Service provider II
The Institute of Photonic Sciences (ICFO)

Location
Barcelona, Spain

Contacts
Scientific contact: Pablo Loza-Alvarez   
Technical contact: Jordi Andilla, Maria Marsal 

Service provider III
Advanced Light Microscopy Facility (ALMF)

Location
Heidelberg, Germany

Contacts
Scientific contact: Rainer Pepperkok
Technical contact: Stefan Terjung